Mendahului Perubaan Moden Testosterone Replacement Therapy 'western medicien' ketinggalan zaman.

Mendahului Perubaan Moden Testosterone Replacement Therapy 'western medicien' ketinggalan zaman.
PATI ASLI kandungan bahan aktif Tongkat Ali Nu-Prep lelaki meningkatkan testosterone, mengurangkan kandungan gula dalam darah, mengurangkan 'STRESS' Tekanan Badan. JESTERU ANDA SIHAT BERTENAGA Tanpa Bantuan Bahan Terlarang / DADAH. NON-DRUG

Sunday, 1 December 2013

FAKTA saintifik terbukti KEJAYAAN BERGANDA Tongkat Ali Nu-Prep lelaki dari kajian klinikal 'USFDA Approved and DOPING Approved' Scientific Discovery - Clinically Proven.



PERHATIAN




 “BANJIR” 2013
 JAGA DIRI 
&
 JAGA KELUARGA, 

Segala yang terbaik itu datangnya dari Yang Maha Esa.

PIHAK KAMI SEJUJURNYA MENDOAKAN SEMUA PIHAK YANG TERLIBAT  “BANJIR”  & BERTUGAS 24/7 TANPA HENTI ‘USAHA MURNI INI’ HANYA YANG MAHA ESA DAPAT MEMBALASNYA, AMIN..
  




‘ANDA PENGGUNA BIJAK’
PEK BARU
NU-PREP LELAKI 


Para  hakim. peguam bela, doktor pakar, anggota beruniform ‘General’ atau berpangkat biasa atau sebaliknya, guru, penyarah, CEO, pengurus besar, pegawai biasa, kakitangan awam, pilot, pemandu, penoreh, pekerja workshop, orang-orang bandar atau di kampung, pengusaha restoran, pelajar universiti, kakaitangan kementerian, jabatn , agensi , pemberita-pemberita, kilnag-kilang akhbar atau majalah, pengurus lading, ahli sukan negara atau biada atau individu, pihak university, kolej, institusi, persatuan-persatuan atau NGO & sebagainya.

Kini sudah tiba masanya, kurang dari separuh dikalangan di sebut diatas yang mengetahui akan Tongkat Ali Nu-Prep lelaki ‘Larut Dalam Air Sepenuhnya / 100%’ melalui proses ‘DIKERING BEKU’. ( water soluble extract – freeze dried ) dipatenkan di Amerika Syarikat ( patent US 7,132,117 B2 ) & Eropah ( Europe Specification Patent EP 1 313 491 B1 ). Produk terhasil dari usaha murni Kerajaan Malaysia & MIT (US). TAPI DIKALANGAN DI SEBUT DIATAS  HAMPIR SEMUANYA TAHU AKAN TONGKAT ALI.
Ini adalah SUASANA yang amat dibimbangi JIKA pengetahuan akan Tongkat Ali berterusan menular pada MINDA PENGGUNA yang menceritakan akan kebaikannya yang pada JANGKA PANJANG memberi keburukkan kepada KESIHATAN TUBUH BADAN. ( contoh kerosakkan buah  pinggang malah organ HATI ).      





Annie George Biortopics Malaysia Berhad 
Journal of Sports Medicine & Doping Studies.
Research Article
The Eurycoma Longifolia Freeze-Dried Water Extract-Physta Does not Change Normal Ratios of Testosterone to Epitestosterone in Healthy Males
PEK BARU 


Corresponding Author :
George Annie
Biotropics Malaysia Berhad, Lot 21, Jalan U1/19
Section U1, Hicom-Glenmarie Industrial Park
40150 Shah Alam, Selangor, Malaysia
Tel: 603-55655600Fax: 603-55655603E-mail: annvalu@yahoo.com

Received August 19, 2013; Accepted September 19, 2013; Published September 26, 2013

Citation: George A, Liske E, Chen CK, Ismail SB (2013) The Eurycoma Longifolia Freeze-Dried Water Extract-Physta® Does not Change Normal Ratios of Testosterone to Epitestosterone in Healthy Males. J Sports Med Doping Stud 3:127. doi: 10.4172/2161-0673.1000127


Copyright: © 2013 George A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract 

Eurycoma longifolia is traditionally known for its adaptogenic properties and often used for the general well-being and increased performance in daily life.

Methods: In a randomized, double blind, placebo-controlled clinical trial, 40 Malaysian men aged 30-55 years performed a battery of physical testings during which the ratio of Testosterone (T) to Epitestosterone (E) was analyzed. The daily intake was 300 mg of the freeze-dried water extract of Eurycoma longifolia root (Physta®, Biotropics Malaysia) or placebo for a period of 12 weeks.

Results: At the end of the study the T/E-ratio in the herbal group ranged from 0.03 to 2.95 and was not significantly different from values at baseline (p=0.49). Further, the ratio-values for E. longifolia compared to placebo did not change over time. The weight lifting force which measures muscular strength using back & leg strength test, increased significantly from baseline to end of the study in the herbal group by approximately 14 kg (p=0.0166).

Conclusion: The ratios of testosterone to epitestosterone did not change during a 12-weeks E. longifolia intake. The data suggest that this herb does not exhibit “doping”- like effects. Instead, muscular strength improved significantly in the back and leg with E. longifolia supplementation rendering this herb good for physical performance minus the doping effects.


Keywords

Eurycoma longifolia; Strength; Quality of life; Doping; Testosterone; Epitestosterone

Introduction


PEK BARU 


It is a common human attitude to want to perform better, particularly in sports and tournaments/competitions. The desire to be better with the use of banned performance-enhancing drugs is considered to be “doping”. Anti-doping rules and testing have been established as an independent and globally accepted standard by the World Anti-Doping Agency (WADA) [1]. Biochemical measurements for the ratio of Testosterone (“T”) to Epitestosterone (“E”) are often used in the fight against doping. The hormones are endogenously built in the body and T increases protein-synthesis resulting in growth of cellular tissue, especially in muscles (“anabolic steroids”), whereas E is an inactive isomer of testosterone. In general, the normal ratio of T to E in urine samples is found similar to be 1:1.




Due to the intake of anabolic steroids or products with similar function, testosterone levels increases while E levels remain stable, resulting in a change in T/E-ratio from normal levels. In 2005, a threshold of T/E-ratio higher than 4:1 (>4) was considered by the WADA as a proof of exogenous intake of anabolic substances [2]. The significance of these measures as well as factors which may influence and possibly facilitate false-positive results have been discussed controversially, e.g., non-steroidal products capable of changing T/E-ratio, specified/nonspecified stimulants, pathological androgen metabolism, individual genetic variations and enzyme polymorphism [1-4].

Adaptogenic substances, including many herbal medicines have been used traditionally with the goal of increasing physical performance. The history of herbs in sports can be traced back to ancient Greek [5]. Famous examples of herbs for sports purposes are among others Panax ginseng, caffeine, and ephedrine and Eurycoma longifolia E. longifolia, also known as “Tongkat ali” or “Malaysian ginseng”, is well-known in Southeast Asian countries and used traditionally for the general wellbeing and better performance in daily living [5-9]. In a randomized, double-blind placebo controlled trial, healthy male subjects on E. longifolia experienced significant improvement in physical functioning, one of the 9 domains found in the SF-36 Quality of Life questionnaire [9]. The question items in this domain were on moderate and vigorous activities, climbing, bending and kneeling, walking, and bathing/dressing, role physical, and vitality. The supplementation with E. longifolia improved strength in physically active male and female seniors between 57 and 72 years of age in an open pilot study and additionally increased muscle mass and fat free mass in men, in another study [10,11]. The ergogenic properties of the herb appear prevalent in these studies. The proandrogenic effect on the laevator ani muscle by an increase in its weight was also seen in rats [12]. It was shown that E. longifolia contains the presence of a 4.3 kDa peptide which increases testosterone levels in rat leydig cells [13]. However, a few clinical data on the effects of E. longifolia in exercise performance are available, such as endurance bicycling and running which still render non-conclusive the effect of E. longifolia in sports performance [6].

Here we report the non-doping effects and the benefits in sport performance during a 12-weeks treatment of Eurycoma longifolia. These data were obtained as an addendum from a randomized, placebocontrolled trial on the quality of life and sexual well-being in men [9].

Methods

Study design and subjects

The randomized, double-blind, placebo-controlled and parallel group study was performed at the Clinical Trial Unit (CTU), Hospital Universiti Sains Malaysia (HUSM), Kubang Kerian, Malaysia. It was conducted in accordance with the Guideline for Good Clinical Practice (ICH-6), Declaration of Helsinki and approved by the Human Research and Ethics Committee for Clinical Studies at HUSM (approval date 24 Dec 2008). An addendum to approve additional endpoints was approved by the Ethics Committee for Clinical Studies at HUSM on 23 Dec 2009 (Ref. USM/PPP/Ethics Com./2009(146)).

One hundred and nine healthy Malaysian men aged between 30 years to 55 years were included. The inclusion/exclusion criteria, randomization and intervention, primary and secondary endpoints as well as sample size estimation and statistical analyses for the study are described in detail in Ismail et al. [9]. Forty subjects agreed for an additional doping test (verum: n=21; placebo: n=19). The subjects were randomly selected and were from the tail end of the ongoing (larger population) study. The additional doping test was added to the protocol at mid-way of the study (larger population) to address possible doping effects upon suggestions by the local Sports Council. The investigators, subjects and study coordinators were double blinded and the products were randomized prior to shipment to the Investigators. The information of the product whether placebo or treatment were sealed in an envelope labeled with the corresponding randomization number and could only is broken upon occurrence of serious adverse event or at the completion of the trial, during data analysis. The envelope containing information of the subjects was checked to ensure there was no breaking of seal.

The subjects were non-athletes and were instructed not to participate in any form of physical training during the study period. A total of four interval visits were scheduled for the entire study: screening (visit 1, week -1 to week-2), measurements taken at baseline (visit 2, day 0: randomisation), at week-6 (visit 3, day 42 ± 3 days) and end-of-study at week-12 (visit 4, day 84 ± 3 days) followed by a followup visit (visit 5 at week-14, via telephone or face to face). At screening (visit 1), clinical examinations and interviews were conducted to select subjects who fulfilled the study criteria. Blood samples were collected from the subjects for routine health check (hematological, renal and liver parameters) and Prostatic Specific Antigen (PSA). At each other visit (V2, V3, V4) Physical Fitness Testings were performed (see Methods 2.3). The primary endpoints for the ratio of hormonal values for Testosterone (T) to Epitestosterone (E) were taken at baseline (V2) and end of the study (V4). The safety was assessed based on documented Adverse Events, physical examinations, clinical and laboratory measures as published [9].

Study medication

Each subject consumed four capsules per day containing either herbal extract or placebo. One capsule contained 75 mg of freeze-dried water extract of Eurycoma longifolia root (Physta®, Biotropics Malaysia Berhad, Kuala Lumpur, Malaysia) as active ingredients, manufactured under Good Manufacturing Process requirements (GMP) with continuous quality control (DER 20:1). Consumption of 500 to 1000 mg root extracts has been reported traditionally, and clinical data show safe consumption of the water soluble extract between 200 to 600 mg [7,14]. Since the present investigation was the first randomized, placebo controlled clinical trial in a large population, a middle range of 300 mg/ day extract was selected. The total daily intake was therefore 300 mg/day herbal extract. The placebo medication (containing maltodextrin) was identical to the active medication however without the herbal extract.

Procedures for the physical fitness tests

Test for physical fitness were performed: each session of the physical fitness tests contained a battery of different types of tests so that various physiological and muscular parameters could be activated/stimulated, finally leading to an improvement of these fitness activities: flexibility- (type of test: Sit & Reach), muscular strength (Hand grip; Back & Leg), muscular endurance (Sit-up & Push up) and body composition (Bioelectrical Impedance Analysis). All these tests were carried out on the same day for each participants and the duration took approximately two hours for each session of five to eight subjects.

Sit and reach: The subjects sat on the floor with legs stretched out straight ahead. They were bare-footed and the soles of the feet are placed flat against the sit and reach box. Both knees were locked and pressed flat to the floor. With the palms facing downwards, and the hands on top of each other, the subjects were told to reach forward along the measuring line as far as possible. Then, the subjects hold that position for at least two seconds while the distance was recorded. The subjects were given three trials and the longest distance to the nearest centimeter reached was noted.

Hand grip: The subjects held the hydraulic hand dynamometer (Jamar, United Kingdom) in the hand to be tested, with the arm at right angles and the elbow by the side of the body. The handle of the dynamometer was adjusted if required and the base was rested on the first metacarpal (heel of palm), while the handle rested on the middle of four fingers. Then, the subjects squeezed the dynamometer with maximum isometric effort, which was maintained for about 5 seconds. No other body movement was allowed. The subjects were strongly encouraged to give a maximum effort. The best result in kilograms from three trials for the dominant hand was recorded, with at least 15 seconds recovery between each effort.

Back and leg strength: The subjects stood upright on the base of the back and leg dynamometer (VacuMed, USA:http://www.vacumed. com/images/12-0403.JPG) with their feet shoulder width apart. They were instructed to hang their arms straight down to hold the centre of the bar with both hands, and with the palms facing toward the body. The chain was adjusted so that the knees are bent at approximately 110 degrees. In this position the subjects’ back are bent slightly forward at the hips, their heads held upright and looking look straight ahead. Then without bending their backs, they pulled as hard as possible on the chain and try to straighten their legs while keeping their arms straight. Additionally, they were told to pull against the weight steadily whilst keeping the feet flat on the base of the dynamometer. The subjects were reminded that maximum performance will result when their legs are almost straight at the end of the lift. The best result in kilograms from three trials was recorded, with at least 15 seconds recovery between each effort.

Sit-up: The subjects lay on a carpeted floor with their knees bent at approximately right angles, with feet flat on the ground. They were instructed to rest their hands on their thighs. Then, they pushed their backs flat and raised high enough for their hands to slide along their thighs to touch the tops of their knees. They were also reminded not to pull with their necks or heads and to keep their lower backs on the floor. They were required to do as many sit-ups as possible in one minute. The number of sit-ups performed in one minute was recorded.

Push-up: The subjects were instructed to use the standard “military style” push-up position with only the hands and the toes touching the floor in the starting position. They were told to lower the chest down towards the floor, always to the same level each time, either till their elbows was at right angles or their chests touch the ground. They were required to do as many push-ups as possible in one minute. The number of push-ups performed in one minute was recorded.

Body composition: A body fat analyzer (Omron HBF 306, Japan) was used to analyze the percent body fat of the subjects. Personal information such as age, gender, weight and height were keyed into the analyzer. Then the subjects were told to grip the device handles and held arms straight out at a 90° angle to the body. Percent body fat, fat mass content and Body Mass Index was calculated automatically in seconds.

Analysis of testosterone T/epitestosterone E

The analysis of urine samples was performed by the Doping Control Centre, Penang, a NATA -accredited laboratory (National Association of Testing Authorities, Australia). The quantification of T/E ratio was determined by Gas Chromatography-Mass Spectrometry (GCMS). The free and conjugated T and E were extracted by solid phase extraction using the Nexus (Varian) column combined with liquidliquid extraction using tert-Butyl methyl ether, followed by hydrolysis using enzyme β-glucuronidase from Esherichia Coli K12 (Roche Diagnostics Mannheim, Germany); extracts were then derivatized with activated N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA). The calibration of T was performed using concentration levels of between 2 ng/mL and 80 ng/mL while the values for E were between 2 ng/mL to 20 ng/mL. Selected ion monitoring mode with electron ionization on the GC-MS was used for identification and quantification of both T and E.

Statistical analyses

Detailed description on the statistical evaluation was already published elsewhere [9]. Data was explored and descriptive statistical analysis was conducted to measure changes in the parameters between E. longifolia and placebo groups at baseline, week 6 and/or end of treatment. Mean (SEM, standard error of the mean) were used for continuous variables of normality distributed data and median (IQR, inter quartile range) for non-normal distribution. The ratio T/E is visualized by Box-Whisker-Plots, showing the spread of the set of data without any assumption of the underlying data distribution. Independent t-test and non-parametric test (as appropriate, Mann- Whitney) were used for comparison of the two randomized groups. Significant changes in the parameters are referred to a p-value of ≤ 0.05.

Results

Forty males agreed for the additional testings: Verum n=21, age (mean ± SD) 45.19 ± 5.37 years (range 30-54 years); Placebo n=19, age 44.63 ± 7.05 years (30-52 years). The flowchart of the study design, bibliographic as well as clinical baseline data of these subjects is listed elsewhere [9].

Hormones

During treatment, hormonal values for Total Testosterone (TT), Free Testosterone (FT), Dehydroepiandrosterone-SO4 (DHEA), the metabolic intermediate in biosynthesis of androgens and Sexual Hormone Binding Globulin (SHBG), the testosterone carrier protein were analyzed for the two treatment groups and are shown in Table 1. There were slight increasing trends in almost all of the values during E. longifolia intake from baseline (V2) to end of study (V4), however, none reached significance: TT median V2-V4: 15.3-16.5 nmol/L (p=0.34), FT 31.4-34.9 pmol/L (p=0.24), SHBG 22.6-25.2 nmol/L (p=0.86). The hormone DHEA increased by 13.6% from baseline (4.2 μmol/L) after 6 weeks (p=0.25), however, at the end of treatment, the value dropped back to the basic value (4.23 μmol/L, p=0.87).

In the placebo-group, FT and DHEA showed increasing values compared to baseline, at the end of trial, (after 12 weeks) (V2-V4: 32.4-36.5 pmol/L; p=0.58) and 3.6-4.5 μmol/L; p=0.62) respectively. Comparisons between groups demonstrated no significant deviations over time for all the hormonal values except for SHBG, which already had a significantly higher starting value in the placebo group at baseline (V2: p=0.03).

Ratio Testosterone to Epitestosterone

After 12 weeks, the T/E-ratio values in the E. longifolia group ranged from 0.03 to 2.95 with a median of 0.2 (n=21) compared to a median (V2) of 0.16 (n=3) at baseline as shown in Figure 1. This would mean no changes occurred in the hormonal relationship during herbal treatment (p= 0.49). At the end of the study, approximately 91% of the subjects had T/E-ratios of up to 1, which is considered to be a “normal” ratio. Similar data were obtained in the placebo group: after 12 weeks, the T/E-ratio values ranged from 0.05 to 3.3 with a median of 0.81 (n=18). Roughly 72% of the subjects had T/E-ratios of up to 1. At baseline, the median value was 0.55 (n=4), demonstrating no changes during treatment (p= 0.82). Between group comparisons showed no statistical differences in the ratios of testosterone to epitestosterone: V2 -EL/placebo: p=0.33; V4 -EL/Placebo: p=0.11). Almost all single values measured in both treatment groups fall within the interquartile range IQR (lower quartile: 25% value and upper: 75% value), except for the minimum and maximum values as well as the 95th percentile value of the T/E ratio in the E. longifolia group (Figure 1).

Physical fitness tests

Flexibility (sit & reach), muscular strength testing: back & leg (kg), hand grip (kg), muscular endurance: sit-up and push-up (times/min) as well as body composition: percent body fat (%) were measured at screening (V1), 6 weeks (V3) and end of treatment visits (V4).

In all the fitness settings no noticeable changes and significant beneficial effects were observed either within treatment groups or between group comparisons, except for back and leg muscular strengths.

The amount of strength the subjects can exert for one repetition during treatment is shown in Figure 2. At baseline (V2), subjects in the E. longifolia group who started with mean ± SEM strength of 128.81 kg ± 5.63 kg (range: 75 kg-196 kg, n=21), increased to 132.26 kg ± 6.39 kg (82 kg-195 kg, n=19) at 6 weeks (V3) and finally reached mean values of 142.78 kg ± 7.95 kg (70 kg-205 kg, n=18) after 12 weeks of treatment (V4). This increase with an approximately 14 kg strength from baseline to end of the study was highly significant with p=0.0166 and from V3 (6 weeks) to V4 (12 weeks) with p=0.0062. In contrast, subjects in the placebo group had a moderate gain (approx. 10.8 kg) in back and leg strength with mean values of 128 kg ± 8.31 kg, n=13 at the end of the trial compared to baseline mean values (117.21 kg ± 5.62 kg, n=19), barely reaching significance (p=0.045).

Discussion



The supplementation of 300 mg per day of Eurycoma longifolia root water extract in healthy middle-aged men, for a period of 12 weeks, was evaluated here for the ratio of Testosterone (T) to Epitestosterone (E) and physical fitness. To the best of our knowledge, this anti-doping measurement, established by the WADA, was applied for the first time in a controlled clinical trial with E. longifolia intake.

In none of the analyzed subjects were the T/E-ratios greater than 4 observed: a threshold which is considered by WADA as an adverse analytical finding requiring further investigations of the individuals. The maximum T/E-ratio of 2.95 was observed after daily intake of 300 mg of E. longifolia water extract. The majority of subjects (>90%) had T/Eratios of up to 1, in the treatment group. The highest ratio for subjects on placebo was 3.3 and more than 72% of the subjects had values of up to 1. Since between-group comparisons showed no noteworthy and statistical significance, the results show that a 12 weeks treatment with 300 mg daily of E. longifolia water extract did not interfere with the normal ratio of testosterone to epitestosterone in middle-aged active and healthy men.

Several other hormones involved in the androgenic metabolism were analyzed in this study: total (TT) and Free Testosterone (FT), Dehydroepiandrosterone (-Sulfate) (DHEA) and sex-hormone binding globulin (SHBG). Although increasing values of these parameters ranging from 7% to 11% could be observed during E. longifolia extract intake, it did not reach significance when compared to baseline or when comparing with the placebo-group. However, literature on E. longifolia states conflicting results on its’ influence in androgenic hormones. It is reported in animal studies that the anabolic action of this herb is in increasing levels of testosterone and human studies suggested that this herb facilitates the production of testosterone by reducing SHBG, thus releasing free circulating testosterone [15,16]. The study, randomized to various doses of standardized E. longifolia extract (200-600 mg daily), showed testosterone and DHEAS at “high normal levels” compared to baseline, in middle aged subjects, after an 8-weeks treatment (statistics were not mentioned in the reference) [17]. In another placebo controlled study, thirty male endurance cyclist, received either 100 mg E. longifolia-water extract (n=15) or placebo (n=15); approximately 30 minutes before and after each of a four 14.91 miles laps, in a highintensity 24-hrs bicycling program [18]. Supplementation of E. longifolia resulted in testosterone levels 16.4% higher while cortisol levels were 32% lower when compared to placebo (p<0.05). The biochemical profile of the cyclists appeared to be in an anabolic hormonal state. In hypogonadic men who have low serum testosterone levels, E. longifolia supplementation was able to significantly increase testosterone levels to normal levels as well [19].

While the role of testosterone is known to affect muscles [20], the role of E. longifolia may not only be limited to testosterone enhancing. In this study, the muscle strengthening effect did not appear to be related to a significant increase in testosterone hormones. This is probably due to E. longifolia purportedly being a natural adaptogenic energizer in the maintenance and healthy ageing in men, suggesting E. longifolia’s role in optimizing testosterone levels only when supoptimal [21]. It can be suggested that E. longifolia supplementation does not indiscriminately increase testosterone levels as do exogenous testosterone supplementation but modulates to optimal levels according to ones’ current hormonal levels. Furthermore, the standardized water extract of E. longifolia (Physta®) contains >22% protein and peptides possibly adding to the ergogenic benefit despite non-significant elevation of testosterone [13,22]. Hence, future studies on the role of the active ingredients in E. longifolia on the contractile properties of the muscles are warranted.

According to recently published reviews on herbs/botanicals including E. longifolia, dietary supplements and herbal medicines, only little conclusive clinical evidence is available to justify the herb’s effects on exercise and sports performance [5,6]. Nevertheless, in this study, the test for muscular strength resulted in significant effects after 12-weeks of Eurycoma-extract intake. There was a highly significant 14 kg improvement (approx. 10% increase) in back and leg strength after 12 weeks (142.78 kg) of E. longifolia intake compared to baseline (128.81 kg; p=0.0166). Placebo-group only reached values of up to 10.8 kg (approx. 8% increase) with a borderline significance of p=0.045. No significant changes in hand grip strength was observed in this study although in physically active male and female seniors aged between 57-72 years of age, increased muscle strength (measured similarly with a hand grip dynamometer) accompanied with significant testosterone elevation, was observed with E. longifolia supplementation of 5 weeks [10]. The ages of the subjects in this study was below 55 years hence the effect of E. longifolia may be more intense in older subjects where sub optimal levels of testosterone normally occurs. An elevation of testosterone in the older subjects may have therefore contributed to the significant improvement of handgrip strength. The precise mechanism attributing to increase in muscular strength is currently inconclusive since in some studies it is accompanied by testosterone elevation and in others, it either did not increase or was not tested. This is particularly important as strength training can provide functional benefits (ergogenic properties): improve general health and well-being as seen in both groups of this study, however, with an enhanced effect present in the E. longifolia group.

There were also other studies demonstrating possible ergogenic properties of E. longifolia. In one randomized placebo-controlled trial, both groups on either 100 mg E. longifolia-extract or placebo, demonstrated a significant increase in muscle strength after 5 weeks of intensive strength training program. However the increase was larger in the treatment (herbal) group compared to the placebo: 6.78% (p=0.006) vs. 2.77% (p=0.011) respectively [11]. Subjects on E. longifolia extract had improved significantly in fat-free mass, fat mass, maximal strength (1-RM) and arm circumference when compared to the placebo group. This data corresponds with data from our study where a similar increase of muscle strength (approximately 10% compared to 6.78%) demonstrated by the back and leg strength test during E. longifolia intake, was observed. This study however did not measure testosterone levels.

The supplementation with E. longifolia did not significantly affect flexibility of the subjects evaluated through the ‘sit and reach’ activity. The mechanism of action for this herb is likely to be proandrogenic whereby testosterone is unlikely to affect the flexibility of subjects [13].

Other studies evaluating endurance were unable to demonstrate the effect of E. longifolia [23,24]. In a previous trial, 12 male athletes (mean age 23.3 years) who received 2 capsules of 75 mg E. longifolia-extract or placebo for 7 days, 1 hour before a 60 min run on the treadmill, showed no changes in running distance and physical responses compared to placebo [23]. In addition, in a separate study, six male cyclists who received a low-dose of E. longifolia medication (0.67 mg), showed no improvement in cycling performance and physical parameters [24]. In this current study, no significant changes were observed in muscular endurance evaluated through Sit-up and Push-ups. In these three situations, it is possible that supplementation of E. longifolia could not affect sports endurance of the subjects which is also dependent on aerobic fitness levels. Furthermore, a short supplementation period (7 days) and low dosage (0.67 mg) of E.longifolia may have also contributed to the lackluster performance of the subjects. In a trial of endurance cyclists supplemented with 100 mg of E. longifolia in a 24-hr mountain biking event, cortisol levels were 32% lower and testosterone levels were 16% higher in supplemented subjects compared to placebo, indicating a biochemical profile that promotes an “anabolic” hormonal state during the intense endurance exercise [18]. The type of exercise which engage different muscle group, anaerobic or aerobic exercise may play a role in the effectiveness of E. longifolia. In the exercise that engaged the larger muscle group such as quadriceps in intense cycling and back muscles in back and leg strength exercises, a beneficial effect of E. longifolia was invoked. Both strength training and intense cycling are primarily anaerobic. Anabolic activity is primarily during anaerobic respiration and was observed in the intense cycling [18]. Hence, it is possible that E. longifolia affects muscle strength in an anaerobic state than endurance which is influenced by aerobic fitness levels such as running and moderate cycling [24,25].

The body fat composition remained unchanged in this study. This was also the case in the larger population [9]. Only in men with BMB>25 kg/m2 was fat loss observed. Overall, the size of the population may be too small to reach statistical significance in other parameters within the physical fitness test and hormonal profiles especially the androgenic hormones. The subjects were also non athletes hence may not actually be physically motivated to be evaluated in physical fitness, leading to poorer performance overall in the fitness test. A population of athletes or recreational athletes could be evaluated in the future to gauge E. longifolia as an effective supplement for sports performance. Additionally, it could be that the study duration of 12 weeks may have been too short and the given daily dose of E. longifolia was not optimal for the desired effect in all physical tests.

Besides ergogenic benefits, the supplementation with E. longifolia may have other beneficial effects, e.g. increase in physical performance, vigor and calmer mood [10,25-28]. An overall clarity of mind and mood elevation accompanied by vigor and muscle strength could be an additional useful attribute in a supplement for sports enthusiast in the future.

Conclusion



The ratio of testosterone to epitestosterone was measured for the first time in healthy males during a 12 weeks intake of 300 mg/day Eurycoma longifolia-extract (Physta®), in a randomized, double-blind and placebo-controlled trial. In both treatment groups, the T/E ratio was normal and below 4. A significant increase in muscle strength was observed for the herbal group compared to placebo in the back and leg strength test though muscular endurance, flexibility and body fat composition remained unchanged with 300 mg daily dose of Eurycoma longifolia over 12 weeks.

Acknowledgements

We would like to thank the phyto-pharmaceutical company Biotropics Malaysia Berhad, Kuala Lumpur, Malaysia, for financial support of the clinical trial.

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  1. Anielski P, Simmchen J, Wassill L, Ganghofner D, Thieme D (2011) Epidemiological investigation of the UGT2B17 polymorphism in doping control urine samples and its correlation to T/E ratios. Drug Test Anal 3: 645-651. 
  1. Stafford ML (2012) Dietary supplements and botanicals in sports: evidence, regulation, and doping controversies. HerbalGram 96: 55-63. 
  1. Chen CK, Muhamad AS, Ooi FK (2012) Herbs in exercise and sports. J Physiol Anthropol 31: 4. 
  1. Goreja WG (2004) Tongkat Ali: The Tree That Cures A Hundred Diseases. Amazing Herbs Press. 
  1. Burkill IH, William Birtwistle; Frederick William Foxworthy; John Brooke Scrivenor; James Gilbert Watson (1966)A Dictionary of the Economic Products of the Malay Peninsula. (2nd Edn) Kuala Lumpur: The Ministry of Agriculture and Cooperatives, Malaysia. 
  1. Ismail SB, Wan Mohammad WM, George A, Nik Hussain NH, Musthapa Kamal ZM, et al. (2012) Randomized Clinical Trial on the Use of PHYSTA Freeze-Dried Water Extract of Eurycoma longifolia for the Improvement of Quality of Life and Sexual Well-Being in Men. Evid Based Complement Alternat Med 2012: 429268. 
  1. Henkel RR, Wang R, Bassett SH, Chen T, Liu N, et al. (2013) Tongkat Ali as a Potential Herbal Supplement for Physically Active Male and Female Seniors-A Pilot Study. Phytother Res .














  1. Hamzah S, Yusof A (2003) The ergogenic effects of Tongkat ali (Eurycoma longifolia): A pilot study. Br J Sports Med 37: 464-470.

  1. Ang HH, Cheang HS (2001) Effects of Eurycoma longifolia jack on laevator ani muscle in both uncastrated and testosterone-stimulated castrated intact male rats. Arch Pharm Res 24: 437-440.

  1. Sambandan TG, Rha CK, Kadir AA, Aminudim N, Saad J, Mohammed M: Bioactive fraction of Eurycoma longifolia. United States



  1. Tambi MIM (2005) Standardized water soluble extract of Eurycoma longifolia (LJ100) on men’s health. Int of J Androl 28: 25-44.












  1. Zanoli P, Zavatti M, Montanari C, Baraldi M (2009) Influence of Eurycoma longifolia on the copulatory activity of sexually sluggish and impotent male rats. J Ethnopharmacol 126: 308-313.


  1. Tambi MI, Kadir AA (2006) Eurycoma longifolia jack: a potent adaptogen in the form of water-soluble extract (LJ100) with the effect of maintaining men’s health Asian J Androl 8: 49-50.



  1. Tambi MI (2009) Nutrients and botanicals for optimizing men’s health. Examining the evidence for Eurycoma longifolia Jack, the Malaysian Ginseng in men’s health. Asian Journal of Andrology 11: 37-38


  1. Talbott S, Talbott J, Negrete J, Jones M, Nichols M, et al. (2006) Effect of LJ100 on anabolic balance during endurance exercise. Journal of International Society of Sports Nutrition 3: S32














  1. Tambi MI, Imran MK, Henkel RR (2012) Standardised water-soluble extract of Eurycoma longifolia, Tongkat ali, as testosterone booster for managing men with late-onset hypogonadism? Andrologia 44 Suppl 1: 226-230.















  1. Herbst KL, Bhasin S (2004) Testosterone action on skeletal muscle. Curr Opin Clin Nutr Metab Care 7: 271-277.


  1. Tambi MI (2005) Standardized water soluble extract of Eurycoma longifolia on men’s health In abstracts of 8th International Congress of Andrology, 12-16 June, Seoul, Korea. Int J Androl 28: 27.















  1. Certificate of Analysis (2008) Freeze dried water extract of Eurycoma longifolia (Physta®). Biotropics Malaysia Bhd.

















  1. Muhamad AS, Keong CC, Kiew OF, Abdullah MR, Lam CT (2010) Effects of Eurycoma longifolia Jack supplementation on recreational athletes’ endurance running capacity and physiological responses in the heat. Int J Appl Sports Sci 22: 1-19.


  1. Ooi FK, Singh R, Sirisinghe RG, Ang B, Jamalullail S (2001) Effects of a herbal ergogenic drink on cycling performance in young cyclists. Malays J Nutr 7: 33-40.


  1. Talbott S, Talbott J, George A, Pugh M (2013) Effect of Tongkat Ali on stress hormones and psychological mood state in moderately stressed subjects. J Int Soc Sports Nutr 10: 28.

  1. Ang HH, Cheang HS (1999) Studies on the anxiolytic activity of Eurycoma longifolia Jack roots in mice. Jpn J Pharmacol 79: 497-500.

  1. Ang HH, Ngai TH, Tan TH (2003) Effects of Eurycoma longifolia Jack on sexual qualities in middle aged male rats. Phytomedicine 10: 590-593.

  1. Ang HH, Lee KL, Kiyoshi M (2004) Sexual arousal in sexually sluggish old male rats after oral administration of Eurycoma longifolia Jack. J Basic Clin Physiol Pharmacol 15: 303-309.











































































































'TheMaking of Tongkat Ali
NU-PREP LELAKI'
KEUTAMAAN ADALAH HALAL

Ketibaan ' Pertemuan bersama doktor pakar ', di hospital 'swasta'





BERMULANYA DI SINI 

FRIM - IBU PEJABAT
MALAYSIA BERJAYA MELAHIRKAN SAINTIS BERTARAF DUNIA. 




KEHADIRAN di sambut segera.



PENGGUNA BIJAK MALAYSIA
ANDA HADIR SEBAGAI GOLONGAN PENDESAK
MAKA JENAMA MALAYSIA PERTAMA MEMENUHI
TENTUTAN ANDA. FOMCA - Federation of Malaysia Consumers Associations 
' PASTIKAN PRODUK MEMPUNYAI KAJAIN DOKTOR PAKAR '



Perbincangan pihak hospital Doktor Pakar Dr Ismail Tambi, Duta Produk Aaron Aziz &
A CEO Biotropics Malaysia Berhad Tengku Sharil Tunku Adnan



Kelebihan produk mempunyai PAKAR / PERUNDING
memenuhi seperti nasihat Kementerian Kesihatan Malaysia
selain PRODUK DIDAFTARKAN & MEMPEROLEHI KELULUSAN.




Mengiriingi DUTA produk, selepas selesai perbincangan bersama PAKAR



KERAJAAN MENYEDIAKAN KELEBIHAN 'KEMENANGAN KEPADA PENGGUNA BIJAK'
MALAYSIA & ANTARABANGSA ( PELANCONG LUAR NEGARA ). ANDA BOLEH MEMBAWA KES INI KE MAHKAMAH JIKA DI DIDAPATI PRODUK IDAK SELAMAT ATAU PALSU.

KEPADA PELANCONG ANTARA BANGSA PROESE YANG LEBIH CEPAT & JIKA TELAH PULANG KE NAGARA ASAL, BERITA AKAN DISAMPAIKAN. 

JIKA DI TIPU PRODUK TIDAK SELAMAT ( MERBAHAYA, LOGAM BERAT, TIDAK LARUT, TIADA BUKTI SAINTIFIK & SEBAGAINYA ) ATAU TERANG-TERANG PRODUK TIRUAN.









KEHADIRAN AARON AZIZI AMAT DINANTIKAN OLEH PIHAK HOSPITAL MALAH PARA PERTUGAS MERASAI TERAMAT BERTUAH PADA HARI TERSEBUT BERSAMA AARON AZIZ DUTA PRODUK TONGKAT ALI NU-PREP LELAKI.




PRODUK TERHASIL DARI USAHA MURNI KERAJAAN MALAYSIA & MIT (US)
BERJAYA DIPATENKAN DI AMERIKA SYARIKAT & EROPAH.



BERSAMA PERTUGAS & PELAWAT ISTIMEWA.






TONGKAT ALI NU-PREP 100 ‘Water Soluble Extract – FREEZE DRIED’.  ( patent US 7,132,117 B2 & Europe Specification Patent EP ,313,491 B1 )


1.      JOURNAL OF INTERNATIONAL SOCIETY OF SPORT NUTRITION. ( BIOMED CENTRAL. THE OPEN ACCESS PUBLISHER ). Research Article. Publication Date : 26 May 2013.

Keywords : Testosterone, Cortisol, Stress, Vigor, Tongkat Ali, Eurycoma, Mood. 

“EFFECT OF TONGKAT ALI ON STRESS HORMONES AND PSYCHOLOGICAL MOOD STATE IN MODERATELY STRESSED SUBJECT.” 








COMPETING INTERESTS.  The authors have no directly competing interests, although one  ( AG ) is an employee of a company that manufactures Tongkat ali extract, and another ( MP ) is an employee of a nutrition company that uses Tongkat ali as one ingredient in an anti-stress dietary supplement. The other  authors ( ST and JT ) conducted this study as employees of Supplement  Watch, which received funding for this trial from Biotropics Malaysia. This study was funded by Biotropics Malaysia and conducted by SupplementWatch.     

Tongkat Ali has been referred to as Malaysia’s “home-grown Viagra” with the Malaysian government investing considerable effort to licence, develop, and sustain research into the potential health benefits of Eurycoma Longifolia through a variety of governmental organizations, including the FOREST INSTITUTE OF MALAYSIA( FRIM ).

Journal of the International Society of Sports Nutrition 2013, 10:28 doi:10.1186/1550-2783-10-28
 Shawn M Talbott (smtalbott@supplementwatch.com)
 Julie A Talbott (jatalbott@supplementwatch.com)


Abstract

Background
 Eurycoma longifolia is a medicinal plant commonly called tongkat ali (TA) and “Malaysian ginseng.” TA roots are a traditional “anti-aging” remedy and modern supplements are intended to improve libido, energy, sports performance and weight loss. Previous studies have shown properly-standardized TA to stimulate release of free testosterone, improve sex drive, reduce fatigue, and improve well-being. 

Methods
We assessed stress hormones and mood state in 63 subjects (32 men and 31 women) screened for moderate stress and supplemented with a standardized hot-water extract of TA root (TA)  or Placebo (PL) for 4 weeks. Analysis of variance (ANOVA) with significance set at p < 0.05 was used to determine differences between groups.

Results 
Significant improvements were found in the TA group for Tension (−11%), Anger (−12%), and Confusion (−15%). Stress hormone profile (salivary cortisol and testosterone) was significantly improved by TA supplementation, with reduced cortisol exposure (−16%) and increased testosterone status (+37%). Conclusion These results indicate that daily supplementation with tongkat ali root extract improves stress hormone profile and certain mood state parameters, suggesting that this “ancient” remedy may be an effective approach to shielding the body from the detrimental effects of “modern” chronic stress, which may include general day-to-day stress, as well as the stress of dieting, sleep deprivation, and exercise training.

( Keywords Testosterone, Cortisol, Stress, Vigor, Tongkat ali, Eurycoma, Mood
CONCLUSION. A wide range of investigations, from laboratory research, to animal feeding studies, to human supplementation trials, have confirmed the health benefits and traditional use of Tongkat Ali root extract. Laboratory evidence shows that eurycoma peptide stimulate release of free testosterone from its binding protein and improve overall hormone profiles. More than a dozen rodent feeding studies have demonstrated improved sex drive, balanced hormonal profile, and enhanced physical function. Human supplementation trials show a clear indication of reduce fatigue, heightened energy and mood, and greater sense of well-being in subject consuming Tongkat Ali root extracts. It is important to note that the majority of these studies, and all of the human supplementation trials, have been conducted on specific hot-water-extract of Eurycoma longifolia ( whisch is the traditional Malaysia preparation ) produced using a patented extraction process to isolate and concentrate the bioactive compound. In conclusion, Tongkat Ali, used for centuries in traditional medicine systems of South East Asia for treating lethargy, low libido, depression, and fatique, appears to have significant potential for restoring hormone balance ( cortisol / testosterone ) and improving psychological mood state in humans exposed to various modern stressors, including aging, dieting, and exercise stress.)      

2.      PHYTOTHERAPY RESEARCH Phytother. Res. (2013) Published online in Wiley Online Library (wileyonlinelibrary.com) DOI: 10.1002/ptr.5017.

ACKNOWLEDGEMENT. This authors wish to thank BIOTROPICS MALAYSIA BERHAD, KUALA LUMPUR, MALAYSIA, for financially supporting this study.


“Tongkat Ali as a Potential Herbal Supplement for Physically Active Male and Female Seniors—A Pilot Study”

Keywords: Eurycoma longifolia; adaptogen; testosterone; ergogenic benef its; increased muscle strength; elderly individuals.







Ralf R. Henkel,1*† Ruxiang Wang,2† Susan Bassett,3 Tao Chen,4 Na Liu,2 Ying Zhu4 and Mohd Ismail Tambi5

1Department of Medical Bioscience, University of the Western Cape, Bellville 7535, South Africa
 2Shenyang Center for Disease Control and Prevention, Shenyang, China 3Department of Sport, Recreation and Exercise Science, University of the Western Cape, Bellville 7535, South Africa
4Liaoning Sport Science Institute, Shenyang, China
5Wellmen Clinic, Damai Service Hospital, Kuala Lumpur 52100, Malaysia.

 Tongkat Ali (Eurycoma longifolia; TA) is known to increase testosterone levels and alleviate aging males’ symptoms. This study aimed at investigating TA as an ergogenic supplement for elderly people. Thirteen physically active male and 12 physically active female seniors (57–72 years) were supplemented with 400-mg TA extract daily for 5 weeks. Standard hematological parameters were taken. In addition, the concentrations of total and free testosterone, dihydroepiandrosterone, cortisol, insulin-like growth factor-1, and sex hormone-binding globulin were analyzed. As additional biochemical parameters, blood urea nitrogen and creatine kinase as parameters of kidney function and muscle damage, respectively, as well as the muscle strength by a simple handgrip test were determined. After treatment, hemoglobin, testosterone, and dihydroepiandrosterone concentrations, and the ratio of total testosterone/cortisol and muscle force remained signif icantly lower in female seniors than in male seniors. Hematocrit and erythrocyte count in male seniors increased slightly but were significantly higher than in female seniors. Treatment resulted in significant increases in total and free testosterone concentrations and muscular force in men and women. The increase in free testosterone in women is thought to be due to the significant decline in sex hormone-binding globulin concentrations. The study affirms the ergogenic benefit of TA through enhanced muscle strength. Copyright © 2013 John Wiley & Sons, Ltd.

3.      ANDROLOGIA. The First International Journal of Andrology.

ORIGINAL ARTICLE

Standardised water-soluble extract of Eurycoma longifolia, Tongkat ali, as testosterone booster for managing men with late-onset hypogonadism?
Keywords :- Adaptogen—Eurycoma longifolia— Hypogonadism—late-onset hypogonadism—testosterone—Tongkat ali













M. I. B. M. Tambi1,2, M. K. Imran2 & R. R. Henkel3
1 Wellmen Clinic, Damai Service Hospital, Kuala Lumpur, Malaysia;
2 Department of Community Medicine, School of Medical Sciences, Universiti Sains Malaysia, Kbg Kerian, Kelantan, Malaysia;
3 Department of Medical Bioscience, University of the Western Cape, Bellville, South Africa

Keywords :- Adaptogen—Eurycoma longifolia— Hypogonadism—late-onset hypogonadism—testosterone—Tongkat ali

Correspondence :- Prof. Mohd Ismail Bin Mohd Tambi, Wellmen Clinic, Damai Service Hospital, Kuala Lumpur 52100, Malaysia.

Tel.: +603 4043 4900 (Ext 791); Fax: +603 4043 5399; E-mail: dr_tambi@yahoo.com Accepted: December 7, 2010 doi: 10.1111/j.1439-0272.2011.01168.

Summary :- In most countries, millions of people are relying on herbal medicines as remedy for numerous ailments. In South-East Asia, Eurycoma longifolia Jack, also known as ‘Malaysian ginseng’ or Tongkat ali, is used to combat stress and disease and to improve physical strength. Moreover, the compounds of the roots of this plant are reported to have aphrodisiac and testosterone enhancing effects in the rat. Considering that human studies are not available, 76 of 320 patients suffering from late-onset hypogonadism (LOH) were given 200 mg of a standardised water-soluble extract of Tongkat ali for 1 month. The Ageing Males’ Symptoms (AMS) according to the standardised rating scale and the serum testosterone concentration were taken. Results show that treatment of LOH patients with this Tongkat ali extract significantly (P < 0.0001) improved the AMS score as well as the serum testosterone concentration. While before treatment only 10.5% of the patients did not show any complaint according to the AMS scale and 35.5% had normal testosterone levels, after the completed treatment 71.7% and 90.8% of the patients showed normal values, respectively. Thus, Tongkat ali extract appears to be useful as a supplement in overcoming the symptoms of LOH and for the management of hypogonadism. 

In conclusion, the standardised water-soluble extract of Tongkat ali proved to be a suitable herbal supplement in overcoming symptoms of LOH and managing hypogonadism. Similarly, men with other causes of hypogonadism may also benefit by taking the herbal extract as an adjunct to their testosterone replacement therapy to enhance their DHEA. Moreover, a treatment like this may be a reasonable therapy for numerous patients, particularly in Third World or emerging countries as such herbal extracts are often cheaper than Western pharmaceutical medicines. In Malaysia, for instance, the supplement is available in most leading pharmacies and popular as a male health supplement. Yet, randomised placebo-controlled studies investigating the positive effects of Tongkat ali on ageing male well-being including its effects on the prostate are missing in the literature thus far and are currently underway.

4.      ORIGINAL ARTICLE. ‘Eurycoma longifolia Jack in managing idiopathic male infertility.’

 Asian Journal of Andrology ( 2010 ) 12: 376-380. Published online 29 March 2010.

Keywords: male infertility, medicinal herbs, semen











CONCLUSION.The results of this study suggest that treatment with the proprietary standardised extract of Eurycoma the percentage of morphologically normal sperm in men with idiopathic  infertility. As spontaneous pregnancies were achieved in 14.7 % of couple in our study and the use of a less invasive, cheaper variety of ART became possible in another 60%, the treatment longifolia Jack significantly improve semen volume, sperm concentration, sperm motility and of such patient with Eurycoma longifolia Jack might be an option in the management of male infertility. In light of the encouraging results of the present study, further studies in a larger group of patients, with follow-up of the outcomes of the ART procedure, are required to elucidate the potential role of Eurycoma longifolia Jack in the treatment of male infertility.

5.      Tongkat Ali Presentation in conjunction with BioMalaysia 2009 Conference and Exhibition.    Nutrients and Botanicals from the Malaysian Rainforest in Optimizing Men’s Health. The Science and Technology behind Tongkat Ali.
Conclusion:- Standardized water-soluble extract of Eurycoma longifolia jack, has undergone numerous Clinical Trials and evaluations locally and internationally and has been found to be a beneficial botanicals optimizing men’s health. The Standardized extract has been patented as a supplement for men’s health specifically in enhancing testosterone and improving male fertility of idiopathic causes. The extract has been found to be non-toxic even at a high dose of 600mg to the body vital functions. Extended studies on the same extract has proven the herb to be an energizer, an aphrodisiac, an ergogenic botanicals which also facilitate muscle fatigue recovery and anti stress. It is a potent adaptogen as well. Overall effects on the various hormones are also positive. It enhanced total testosterone and DHEA (Dehydroepiandrosterone) which also modulate testosterone level, ensuring enough free testosterone for the body’s need. Its other adaptogenic effects include positive effect on body cortisol levels as well as on growth hormone as it modulates the released of IGF-1. With all these ample scientific and clinical findings, Standardized patented water-soluble extract of Eurycoma
longifolia, has emerged as a potent botanical supplement in men’s health. # This article is published in the ASIAN JOURNAL OF ANDROLOGY (Volume 11, Supplement 1, October 2009)





STATUS AKTIVITI  TERKINI
TAHAP INTERLEKTUAL
Berkaitan 'paten'
oleh Pengguna Malaysia
PENGGUNA TIADA KEYAKINAN DIRI

Berkaitan 'paten', Pengguna Malaysia lebih berminat produk dari luar sama ada kakitangan kerajaan & atau swasta. 

KETAHUILAH, kelebihan dari sudut 'SIHAT BERTENAGA TANPA BAHAN TERLARANG / DADAH' meningkatkan testosterone - hingga kini BELUM ADA SEBARANG produk yang berjaya meningkatkan testosterone dalam tempoh singkat 24 jam. ( tercatat pada kajian klinikal ).

Tongkat Ali Nu-Prep Lelaki berjaya meningkatkan hingga 16.5%.  'BUKAN SEPERTI UBAT DARI FARMASI' ( jika dari farmasi sudah pasti campuran kimia / DADAH, bakal menghadapi RISIKO BURUK JANGKA PANJANG ). Peningkatan testosterone ini tidak memberi sebarang RISIKO BURUK kepada kesihatan tubuh badan. Kebaikan & keberkesanan kepada kesihatan di jamin SELAMAT, BERKESAN & BERKUALITI. KEHEBATAN ini seiring kriteria WHO. 

TONGKAT ALI, eurycoma longifolia JACK, long jack 
Kehebatan Tongkat Ali Nu-Prep lelaki kelebihan mendahului perubatan barat
( western medicine ) SIHAT BERTENAGA TANPA BAHAN TERLARANG / DADAH.

( US PATENT 7,132,117 B2 & Europe Specification Patent EP 1 313 491 B1 )

Untuk pengetahuan semua pihak BAHAWA TONGKAT ALI NU-PREP LELAKI adalah produk terlaris di farmasi. ( fakta ANUGERAH ini sebagai produk terlaris MENDAHULUI PERUBATAN BARAT - 'western medicine', atau sebarang bentuk ubat / supplement dari negara barat BUKAN DARI NEW ZEALAND, EROPAH ATAU US ) SEKALI LAGI YANG PASTI HANYA TONGKAT ALI NU-PREP LELAKI. JENAMA MALAYSIA YANG TERBAIK & TERBUKTI.

MUNGKIN fakta ini dapat memberi semangat baru menjelang 2014. Kehebatan Tongkat Ali Nu-Prep lelaki mendapat tarikan PEMBELI DI FARMASI DI SELURUH MALAYSIA SEBAGAI PRODUK UNTUK KESIHATAN PALING LARIS. ( tidak produk-produk lain yang 'CAKAP TIDAK SERUPA BIKIN'-TANPA BUKTI / FAKTA SOKONGAN' ).








Mona.vie telah menggunakan 'extract' Tongkat Ali dari proses yang sama untuk pasaran di Amerika Syarikat.

Kajian klinikal terbukti. Kehebatan 'extract' Tongkat Ali sebagai bahan aktif yang sama terkandung pada Tongkat Ali Nu-Prep lelaki telah membawa JENAMA MALAYSIA menembusi pasaran luar di US ( Amerika Syarikat ). Kajian terkini dibiayai oleh pihak Biotropics Malaysia Berhad telah menghasilkan satu 'scientific discovery - clinically proven'. Pihak mona.vie TELAH MENDAPATI BAHAWA KEHEBATAN KEBERKESANAN KEPADA WANITA TERBUKTI MENGURANGKAN 'STRES' ( STRESS REDUCER ) malah ia adalah baik untuk 'stress, mood, vigor. 



 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"


NU-PREP LELAKI
 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"

NU-PREP LELAKI
 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"

NU-PREP LELAKI
 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"

NU-PREP LELAKI 
 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"

NU-PREP LELAKI
 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"


Mukjizat Bergelar Tongkat Ali

Professor Emeritus Dr.Mohd Ismail Tambi. ( http://www.facebook.com/dr.ismail.tambi )
Klinik Sejahtera Lelaki
Damai Service Hospital
Jalan Ipoh

NU-PREP LELAKI



Tongkat Ali atau nama saintifiknya Eurycoma longifolia jack ialah herba yang biasa didapati dihutan belentara, Nausantara berdekatan lereng pergunungan. Namanya yang dikaitkan dengan lelaki tampan yang berdarah pahlawan sememangnya memberi erti mukjizatnya yang terselindung, iaitu membantu kesihatan,  kesejahteraan dan semangat kelelakian seperti  tongkat yang membantu mengangkat mereka yang lemah.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI

Pohon ini dikenali sebagai penawar pahit kerana ianya amat pahit dan mujarab untuk pelbagai masalah kesihatan dan penyakit. Ia juga dikenali sebagai pasak bumi kerana akarumbinya yang  berjelar lurus kedalam bumi dan kukuh dan amat sukar untuk dicabut. Terdapat beberapa spesis Tongkat Ali, tapi spesis yang mengandungi banyak ramuan perubatan ialah dari spesis  Longifolia dan Apiculata.Mengikut Professor Arif Adimoleja dari Universiti Surabaya,  Indonesia, spesis Tongkat ali dari Semenanjung Malaysia mengandungi lebih banyak ramuan kesihatan, terutama sekali ramuan yang membantu tenaga batin lelaki jika dibandingkan dengan spesis yang terdapat di Indonesia atau Negara Asia yang lain.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LALAKI

Ini mengikut beliau ada kaitan dengan khasiat yang terdapat dalam bumi Semenanjung Malaysia. Khasiat perubatan Tongkat ali terkandung dengan banyak dalam akarumbinya. Ia mengandungi pelbagai alkaloid yang mujarab sebagai penawar untuk demam kura ( Malaria ), penawar penyakit alahan  ( allergy ), mencegah demam, mengandungi elemen nyah kanser, mengandungi ( SOD ) Superoxide dismutase yang berfungsi sebagai antioxidant, anti rheumatic ( mengurangkan kesakitan otot dan sendi akibat rheumatic ), fungsi aphrodisiac  atau ubat perangsang shaywat dan  ubat melewatkan proses penuaan. Oleh kerana ianya pahit, kadangkala ia digunakan sebagai bahan perisa untuk kopi dan minuman pahit. Jadi kandungannya dalam kopi atau teh ialah semata mata untuk perisa dan tiada apa apa khasiat yang lain kerana jika kandungannya lebih memang tidak dapat meminumnya kerana ia teramat pahit!
                         IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI

Pada zaman melayu purba, kikisan akar Tongkat Ali biasa digunakan sebagai campuran dalam teh atau kopi dan minuman ini biasa diamalkan oleh orang Nausantara dan Melayu untuk tenaga, keselesaan dan kesegaran. Pendatang dari Negara barat saperti orang Inggeris dan Portugese mula memberi perhatian tentang herba itu apabila mereka mula mengkaji perubatan tradisional melayu. Koleksi maklumat yang mereka cuba membukukan banyak mengupas pelbagai herba yang penuh mukjizat yang mana Tongkat ali mendapat perhatian. Ini membuatkan herba Tongkat ali terkenal sebagai herba yang dapat membantu memantapkan kesejahteraan kaum lelaki.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
Kajian awal yang mengunakan akstrak larutan alcohol dan chloroform didapati amat toxic tapi mujarab untuk menangani masalah kesihatan saperti deman kura dan jangkitan kuman dan virus. Kesan toxic kepada hati dan buah pinggang membuatkan akstrak tersebut tidak sesuai untuk kegunaan manusia. Akstrak larutan air pula didapati selamat apabila prosesnya diubahsuai untuk mengeluarkan bahan toxic.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
Kajian awal kepada tikus menunjukkan yang extrak larutan air Tongkat Ali yang mengandungi elemen glycoprotein dapat meningkatkan hormone Testosterone 4 kali ganda. Kajian kepada tikus tua dengan akstrak Tongkat ali larutan air membuatkan tikus tua aktif dan gresif. Akstrak ini tiada kesan toxic kepada hati dan buah pinggang dan kajian klinikal kepada manusia dijalankan pada penghujung 1999 dibeberapa buah Pusat Pengkajian Perubatan tempatan dan antarabangsa dan hasil dari kajian tersebut akstrak Tongkat ali larutan air yang telah menjalani pelbagai proses piawayan kini telah mendapat iktiraf patent pada 2004 ( US patent: US2004/0087493 A1 ) ( terkini ‘United States - patent US 7, 132,117 B2 & Europe Specification Patent EP 1,313,491 B1’  )  oleh syarikat Biotropics Malaysia Berhad , ( syarikat berkaitan kerajaan – GLC  ) dengan jenama  terkini Nu-Prep 100 )
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
Menyusuli kajian Biomedikal dan Medikal yang pernah dilakukan kepada akstrak tersebut, hanya akstrak ini sahaja yang telah menjalani pelbagai ujian dan penyelidikan yang komprehensif yang membuatkannya akstrak Tongkat ali yang premium. Beberapa kajian yang ‘landmark’ yang pernah dijalankan kepada akstrak ini ialah seperti  berikut:
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
KAJIAN AWAl mengunakan tisu buah zakar manusia yang dikultur dengan akstrak tersebutr mendapati peningkatan testosterone dan androsteinedione ( androgen saperti testosterone ) ketahap tiga kali ganda normal. Tiada peningkatan tahap dihidrotestosterone ( ada kaitan dengan kejadian kanser kelenjar prostat ). Penemuan ini seolah olah membuka jalan untuk kajian klinikal kepada manusia dilakukan tanpa was was. Kajian klinikal yang seterusnya dijalankan disebuah Pusat Latihan Sukan di Kansas, USA dengan 10 orang penunggang basikal mendapati yang tahap hormone testosterone meningkat dengan pengambilan 200mg akstrak tersebut.
  IQRA, Bacalah....
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KAJIAN SUSULAN disebuah Pusat Perubatan di Kuala Lumpur melapurkan peningkatan hormone testosterone dan hormone DHEA sekali gus dengan penurunan SHBG ( Ikatan protin globulin dengan molekul testosterone ) dan peningkatan hormone testosterone yang bebas ( testosterone bebas ikatan yang boleh digunakan badan ) dengan pengambilan 200mg akstrak tersebut.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
Kajian yang dilakukan diFakulti Perubatan Bahagian Sains Sukan dengan sekumpulan atlit yang mengambil 200mg akstrak yang sama juga menunjukkan yang atlit tersebut dapat membina kekuatan tenaga otot dan penurunan lemak badan yang mana kedua duanya ada kaitkan dengan kesan androgen.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
KAJIAN KLINIKAL yang komprehensif tentang kesan akstrak premium itu kepada 20 lelaki mendapati akstrak itu tidak memberi apa apa kesan negative kepada profil darah, tidak member kesan negative kepada buah pinggang dan hati dan meningkatkan tahap hormone testosterone, DHEAS dan mengoptimumkan hormone tiroxin dan hormone ketumbesaran IGF-1. Akstrak ini juga dapat meningkatkan tahap kolesterol baik dalam darah dan tidak member apa apa kesan kepada tekanan darah atau urat saraf walau pun diambil dengan dos yang tinggi.
 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
Kajian susulan yang dilakukan di USA oleh Professor Talbott kepada atlit penunggang bisikal mendapati akstrak tersebut dapat menambahkan testosterone sebanyak 16.5% dalam masa 24 jam dan mengurangkan tahap hormone stress iaitu cortisol sebanyak 33% dalam masa 24 jam juga. Kajian ini jelas menunjukkan kemujaraban aksdtrak premium ini meningkatkan testosterone dan mengurangkan tekanan badan.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
Kajian yang sama yang dijalankan oleh Professor Ruxiang Wang diLioning, China kepada beberapa atlit berumur yang mengambil 200mg akstrak tersebut. mereka rasa diri mereka lebih aktif dan agresif dengan peningkatan tahap hormone ketumbesaran dan testosterone serta mereka dapati proses pemulihan lenguh otot setelah melakukan senaman berkayuh itu cepat.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
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Kajian yang dilakukan kepada lelaki yang mengalami masalah sperma dengan pengambilan akstrak premium tersebut mendapati peningkatan kepada bilangan dan keaktifan sperma dengan sekali gus peningkatan kualiti sperma yang dapat menghasilkan kehamilan spontan sebanyak 17%.
  IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI
Banyak lagi kajian klinikal dan biomedical sedang dijalankan kepada akstrak tongkat ali premium ini untuk meningkatkan lagi keyakinan diri para pengamalnya yang sudah sedia maklum yang Tongkat ali ini bukan sekadar memantapkan fungsi shaywat lelaki yang biasa diwawarkan oleh tukang ubat tradisional, tapi ia lebih dari itu, iaitu memantapkan kesihatan, mengurangkan tekanan, mempunyai kebaikkan untuk kesuburan lelaki serta memantapkan kesejahteraan lelaki. Ia benar benar suatu mukjizat untuk lelaki.

 IQRA, Bacalah....
"MUKJIZAT BERGELAR 
TONGKAT ALI
 NU-REP LELAKI"
NU-PREP LELAKI

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